Pseudomonas mosselii is a Gram-negative, rod-shaped, bacterium clinically isolated in France.
HB13667 was associated with a strain of Bacterial strains used in this work are listed in Table Chromosomal DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega, USA).
For scanning electron microscopy, tissues were fixed in cacodylate-buffered 3% glutaraldehyde, dehydrated in increasing concentrations of acetone (30, 50, 70, 95, and 100%), critical-point dried, gold sputter-coated and analyzed in a scanning electron microscope (Quanta 200; FEI, Eindhoven, The Netherlands), using high vacuum mode.Analysis of intercellular junctions was carried out by immunofluorescence with specific anti-zonula occludens 2 or anti-desmoplakin primary antibodies, and FITC-labeled secondary antibodies. Feuilloley. Overnight bacterial cultures were spread on 240 mm × 240 mm LB plates, air dried in a laminar flow and then discs containing antibiotics were placed on the LB plates. HB3267 (as well as HB4477, HB4557, and HB3536) harbor an 80 Kb megaplasmid, pPC9, previously shown to be required for antibiotic resistance (Many pathogenic bacteria are highly resistant to antibiotic treatment, and one important antibiotic resistance mechanism is the formation of biofilms (The histological analysis of human skin fragments incubated in the presence of the different bacteria strains showed that all strains were able to alter the structure of both the epithelium and the stromal layer of the human skin, this was particularly notable for strains HB8234, HB4184, and HB3267. Int.
2002. Pseudomonas mosselii sp. Taken together, these data reveal that HB4184 has a lower pathogenic potential than HB3267 or HB8234, however, its ability to form biofilms, which is superior to that of Strain HB13667 did not show pathogenic traits in any of the biological systems used in this study. These include P aeruginosa, P fluorescens, P putida, P cepacia, P stutzeri, P maltophilia, and P putrefaciens. 4. 52:363–376 . Immunohistochemical detection of laminin, which is one of the most important components of the basement membrane of the epithelium, showed significant alterations of the dermal-epidermal junction of tissues incubated with the different strains, as shown in Figure When two specific proteins with a key role in cell-cell attachment (ZO-2 and desmoplakin) were analyzed by immunofluorescence, we found that the signal intensity of both components of the cell-cell junction complex were reduced after exposer to the different To determine the immune response initiated by the presence of the different strains Infections caused by multidrug-resistant Gram-negative bacteria have become a growing challenge in hospital environments; in fact these microorganisms cause a higher number of nosocomial infections than resistant Gram-positive bacteria (methicillin-resistant To explore putative phenotypic differences among environmental and clinical isolates, the well-known environmental strain Taken together, data from this study, have revealed the variability in the pathogenic traits exhibited between the Among the clinical strains tested the highest pathogenic potential was associated with HB3267, which exhibited pathogenic traits in almost all of the assays: it had a cytotoxic effect on human skin cells, it caused damage to the epithelial layer, and it provoked an immune-system response.
In all cases, we found vacuolization and nuclear degeneration of epithelial cells along with dermal disorganization when compared to control human skin (Figure In order to determine if the strains were capable of disrupting the epithelial layers, we tested important structural proteins of the skin using immunohistochemistry and immunofluorescence. D'Andrea M, et al. It did not cause damage on the skin of Wister Rats and did not affect insect survival. References External links. When inoculated into wounds on rats HB3267 caused severe damage to the epidermis and the underlying connective tissue. Microbiol. Finally, it showed a deleterious effect on the survival of HB3267 (or variants of this strain) were isolated from four different patients at the Besançon Hospital, the rest of strains tested were isolated only once. Prosthetic valve endocarditis caused by A. Chapalain, G. Rossignol, O. Lesouhaitier, A. Merieau, C. Gruffaz, J. Guerillon, J.-M. Meyer, N. Orange, and M.G.J. Results (Supplementary Table S1) showed two distinct antibiotic resistance profiles: (a) clones resistant to a high number (around 84%) of the antibiotics tested, this was the HB3267 strain (the same profile was seen with HB4477, HB4557, and HB3536). J. Microbiol. This finding could be linked to its innate resistance to most of the antibiotics tested, since antibiotic resistance is considered a key factor in nosocomial prevalence (Strain HB8234 revealed pathogenic potential on human skin tests, where it caused cytotoxicity and histological disorders; in addition, it further damaged the injured skin of Wistar rats, but it did not affect larvae survival.
HB8234 was also sequenced recently by our research group (This strain showed pathogenic traits, but only on human skin, where it caused a moderate cytotoxicity, histological damage and triggered an immune response. Comparative study of 7 fluorescent pseudomonad clinical isolates. PCR amplifications were performed according to standard procedures using Euro Taq polymerase (EuroClone, Italy).16S rDNA amplification was performed using the F8 and R798 primers (Multilocus sequence typing was carried out using a set of primers (listed in Supplementary Table S2) to amplify the complete sequence of five housekeeping genes: RNA polymerase sigma factor Antibiograms were performed with commercial discs (Biomerieux); 31 different antibiotics were tested. 2002. 2002. Briefly, tissue sections were deparaffinized, incubated in pH 6 citrate buffer for 40 min at 95°C for antigen retrieval, and primary antibodies were applied for 60 min at room temperature. Samples were analyzed using a confocal microscope. Name Abstract for Pseudomonas mosselii Dabboussi et al. Secondary antibodies were applied and the reaction was developed using a commercial 3-3′ diaminobenzidine kit (Vector Laboratories, Burlingame, CA, USA). Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas, Pseudomonas mosselii Dabboussi et al. The species Pseudomonas mosselii was originally described and validly published by Dabboussi et al.