Eight strains of acidophilic bacteria, isolated from mine-impacted and geothermal sites from different parts of the world, were shown to form a distinct clade (proposed genus “We use cookies to help provide and enhance our service and tailor content and ads. Alicyclobacillus acidoterrestris is a soil-borne, thermo-acidophilic, endospore-forming bacterium which causes the spoilage of pasteurized fruit juices with the production of guaiacol taints. Proposal for the creation of a new genus Purification and amino acid sequence of two small, acid-soluble proteins from Cloning and nucleotide sequence of three genes coding for small, acid-soluble proteins of Improving the specificity of high-throughput ortholog predictionDating of the human-ape splitting by a molecular clock of mitochondrial DNAInvestigation of factors influencing spore germination of Evolution of ribonuclease H genes in prokaryotes to avoid inheritance of redundant genesStructure of a protein-DNA complex essential for DNA protection in spores of Biorepositories and their foundations—microbial forensic considerationsCloning and nucleotide sequencing of genes for small, acid-soluble spore proteins of Small, acid-soluble, spore proteins and their genes from two species of A method for release and multiple strand amplification of small quantities of DNA from endospores of the fastidious bacterium A gene encoding a small, acid-soluble spore protein from alkaliphilic Database resources of the National Center for Biotechnology InformationPurification and characterization of additional low-molecular weight basic proteins degraded during germination of Cloning and nucleotide sequencing of genes for the second type of small, acid-soluble spore proteins of A proposed syntax for Minimotif Semantics, version 1Comparison of various properties of low-molecular weight proteins from dormant spores of various We have applied a new method of searching for sspE genes, which now appear to also be absent from a clade of Bacillales species that includes Alicyclobacillus acidocaldarius and Bacillus tusciae. Alicyclobacillus acidocaldarius (Darland and Brock, 1971) Wisotzkey et al., 1992 emend. . Introduction 245 Thermophilic acidophilic bacteria in foods and beverages 247 Alicyclobacillus 249. Assembly members: Alicyclobacillus acidocaldarius thioredoxin, polymer, 105 residues, 11475.0 Da. The pure enzyme was used to compare the EST2 hydrolytic activity to the activity of endogenous esterase EstA from Lactococcus lactis.The results indicate that EST2 exhibits 30-fold-higher esterase activity than EstA. γ-Type small, acid-soluble spore proteins (SASP) are the most abundant proteins in spores of at least some members of the bacterial order In the current work, we have examined genome sequence information for spore-forming The dried acetic acid extracts from various types of spores were dissolved in 30 μl of 8 M urea plus 15 μl diluent for acid-acrylamide gel electrophoresis, samples were run on acrylamide gel electrophoresis at a low pH, and the gels were stained with Coomassie blue (Comparison of amino acid sequences of γ-type SASP from spore-forming Comparison of amino acid sequences of α/β-type SASP from In order to better understand the genetic history of The sequences of γ-type SASP were updated and extracted to a relational database containing over 3 million NCBI proteins from completed proteomes and placed in a smaller data mart containing proteins in the 58 fully curated genomes of Comparison of amino acid sequences of SASP-γ obtained from completed genomes of spore-forming Polyacrylamide gel electrophoresis at low pH of acetic acid extracts from spores of The N-terminal amino acid sequence of the protein in band 2 was PNQGGSXN, and this sequence matched that of a protein, termed Ppo, encoded in the Putative upstream and downstream regulatory regions for genes encoding α/β-type and γ-type SASP from various species. These were used as query sequences to search the genome scaffolds of the ... United Kingdom) and viewed with a Leitz Labolux phase-contrast microscope at a magnification of 400×. Alicyclobacillus species Alicyclobacillus acidocaldarius Name Synonyms Bacillus acidocaldarius Darland & Brock, 1971 Homonyms Alicyclobacillus acidocaldarius (Darland & Brock, 1971) Wisotzkey et al., 1992 Bibliographic References