To obtain J Am Chem Soc. Front Microbiol. Metabolite induction via microorganism co-culture: A potential way to enhance chemical diversity for drug discovery. Image credit: Zachary S. Randall (Florida Museum of Natural History, Gainesville, FL).Models to predict six life outcomes based on data collected over 15 years suggest that the use of predictive models in criminal and social systems may need to be reevaluated, along with the assumption that life trajectories can be predicted.Therapeutic applications of transcription factor decoy oligonucleotidesA gene therapy strategy using a transcription factor decoy of the E2F binding site inhibits smooth muscle proliferation in vivoTargeted inhibition of Stat3 with a decoy oligonucleotide abrogates head and neck cancer cell growthDual blockade of cyclic AMP response element- (CRE) and AP-1-directed transcription by CRE-transcription factor decoy oligonucleotide: gene-specific inhibition of tumor growthThe stability of different forms of double-stranded decoy DNA in serum and nuclear extractsCytoplasmic deposition of NFκB decoy oligonucleotides is insufficient to inhibit bleomycin-induced pulmonary inflammationE2F decoy oligodeoxynucleotides effectively inhibit growth of human tumor cellsRegulation of secondary metabolism in streptomycetesDiversity and biogeography of marine actinobacteriaThe ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) plays a conditional role in antibiotic production and morphological differentiationRegulation of the Streptomyces coelicolor calcium-dependent antibiotic by absA, encoding a cluster-linked two-component systemGenetic analysis of absB, a Streptomyces coelicolor locus involved in global antibiotic regulationTranscriptional regulation of the redD transcriptional activator gene accounts for growth-phase-dependent production of the antibiotic undecylprodigiosin in Streptomyces coelicolor A3(2)Transcriptional activation of the pathway-specific regulator of the actinorhodin biosynthetic genes in Streptomyces coelicolorPCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosminThe role of the novel Fem protein VanK in vancomycin resistance in Streptomyces coelicolor Although iron-restricted culture conditions triggered actinorhodin production, the amount synthesized was markedly lower, and the produced actinorhodin was rarely excreted compared with that under coculture conditions (Fig. J Ind Microbiol Biotechnol. Search worldwide, life-sciences literature Search. ClueGO: a cytoscape plug-in to decipher functionally grouped gene ontology and pathway annotation networks. ZX executed the experiments. NNF10CC1016517).Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, 34141, Republic of KoreaNamil Lee, Woori Kim, Jinkyoo Chung, Yongjae Lee, Suhyung Cho, Sun Chang Kim & Byung-Kwan ChoBiomedical Omics Group, Korea Basic Science Institute, Cheongju, 28119, Republic of KoreaDivision of Bio-Analytical Science, University of Science and Technology, Daejeon, 34113, Republic of KoreaIntelligent Synthetic Biology Center, Daejeon, 34141, Republic of KoreaDepartment of Bioengineering, University of California San Diego, La Jolla, CA, 92093, USADepartment of Pediatrics, University of California San Diego, La Jolla, CA, 92093, USANovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, 2800, DenmarkYou can also search for this author in Type II polyketide backbone biosynthesis and branched-chain amino acid degradation pathways in Taken together, based on the analysis of transcriptome changes in the two species, we found that Notably, during coculture, enhanced production of actinorhodin by To address the iron competition between the two species, we measured extracellular and intracellular iron levels.
Genomic basis for natural product biosynthetic diversity in the actinomycetes. You can also search for this author in Their utility has been demonstrated mostly in eukaryotic systems, where a spur to their development was their potential to function as novel classes of therapeutic agents (We used decoy oligonucleotides to study the regulation of the blue-pigmented antibiotic actinorhodin in Perhaps as a consequence of the complex regulation of antibiotic production, many pleiotropic mutants identified by genetic screens are conditional; for example, the antibiotic nonproducing phenotype of a DNA–protein interactions controlling expression of Because our aim was to discover regulatory elements involved in the repression of T7 exonuclease/DNase I mapping of candidate regulatory motifs within the promoter of We developed a rapid agar-plate-based assay to determine whether the decoys had any effect on antibiotic production. Communicated by Melvin I. Simon, California Institute of Technology, Pasadena, CA, November 16, 2007 (received for review April 10, 2007)We have adapted and extended the decoy oligonucleotide technique for use in prokaryotes.