The genus Cronobacter has undergone extensive diversification during the course of its evolution, with some species clearly pathogenic for humans and other species still with unknown or uncertain impact on human health. In the case of CS the biovolume of the biofilm was 91,387.6 ± 5,346.1 µmThe percentage of dead bacteria (calculated as the biovolume of dead bacteria with respect to total biovolume) in control biofilms (untreated) was 0.342 ± 0.138% (Percentage of dead cells relative to total cells in biofilms formed by 48-hour-old biofilms of Percentage of dead cells relative to total cells in biofilms formed by 48-hour-old biofilms of In view of the scant loss of viability after treatment with PAA or BZK at 1MIC, it may be surmised that the anti-biofilm effect of these treatments is linked principally with the shedding of cells (considerable in the case of The highest percentages of dead cells were observed after treatment with PAA at 2MIC when It is concluded that exposure to increasing sub-inhibitory concentrations of SHY, PAA or BZK triggered adaptive tolerance of these compounds in strains of The biocides studied were sodium hypochlorite (10% of active chlorine; SHY, Sigma-Aldrich Co., St. Louis, Missouri, U.S.A.), peracetic acid (39% solution of peracetic acid in acetic acid; PAA, Sigma-Aldrich) and benzalkonium chloride (BZK, Sigma-Aldrich).
We conducted a multicenter study to assess the occurrence of C. sakazakii across Europe and the extent of clonality for outbreak detection. The minimum inhibitory concentration (MIC) of citral against C. sakazakii was determined via agar dilution method, then Gompertz models were used to quantitate the effect of citral on microbial growth kinetics. isolates were pan-susceptible, continued antibiotic resistance … The broader occurrence of Cronobacter in food ingredients, finished products, and food manufacturing environments is covered. e epidemiological OH, AA, IH, MK, AA, SF participated in the study design, Arlet G, Brami G, Décrè D, Flippo A, Gaillot O, Lagrange PH, ment length polymorphism of TEM beta-lactamases. The 45 isolates included four species: C. sakazakii (n = 37), C. malonaticus (n = 3), C. dublinensis (n = 3), and C. muytjensii (n = 2); that presented 20 different fusA alleles.
We here evaluated the MALDI Biotyper (Bruker Daltonics) for the identification of fastidious Gram-negative rods (GNR). EFSA. & Huang, L. Growth kinetics and model comparison of EFSA & ECDC. You can also search for this author in Even though the results of this study showed that Cronobacter spp. Unable to load your collection due to an error MLST analysis revealed 32 sequence types (STs), 13 of which were newly identified. in foods, especially due to their highlighted association with neonatal infections through the ingestion of reconstituted powdered infant formula (PIF). This organism was first characterized in 1929 as a ‘yellow-pigmented coliform’ and was discovered to be the causative agent of septicemia in infants. This concern resulted in improved microbiological criteria recommendations by the Codex Alimentarius Commission and revised WHO advice on the preparation of infant feeds. Despite the high clonality of Cronobacter , PFGE profiles differentiated strains across the sequence types into 15 pulsotypes. A surveillance study was designed to identify antibiotic resistance among clinical Cronobacter spp. 2017 May;63:129-138. doi: 10.1016/j.fm.2016.11.011. The disease is associated with a rare cause of invasive infection infants with historically high case fatality rates (40–80%). A surveillance study was designed to identify antibiotic resistance among clinical Cronobacter spp. If a patient is colonized, rather than infected, with Cronobacter, treatment is not needed. This gram-negative enteric bacillus has been implicated most often in neonatal meningitis and sepsis and has only rarely been isolated in adults. previous Cronobacter research, this study is mainly focused on C. malonaticus and is aimed to investigate its virulence characteristics that enable this species to cause adult and neonatal infections. After incubation, inhibition zones were measured and the strains were classified as susceptible, intermediate (with reduced susceptibility) or resistant on the basis of CLSI criteriaThe structure of the biofilms was investigated using a method described in a previous publicationTo view the biofilms a Nikon Eclipse TE 2000-U confocal scanning laser microscope (CLSM) was used with the EZ-C13.60 program (Nikon Instruments Inc., New York, U.S.A.). The aim of this study was to detect Cronobacter spp.
The discs were placed on the MH plates which had previously been inoculated with the strains and were incubated for 24 hours at 30 °C. Free PMC article Each spot was analysed in triplicate (24spec-tra in total). Carpentier, B. Unfortunately, information on the diversity, pathogenicity, and virulence of Cronobacter species obtained from various sources is still relatively scarce and fragmented. All strains were sensitive to all antibiotics (n = 10) tested. have been recognized as causative agents of various severe infections in pre-term or full-term infants as well as elderly adults suffering from serious underlying disease or malignancy. A.
A total of 15 antibiotics (Oxoid) were tested: AMP, ampicillin (10 µg); TE, tetracycline (30 µg); CIP, ciprofloxacin (5 µg); C, chloramphenicol (30 µg); SXT, trimethoprim/sulfamethoxazole (25 µg); NA, nalidixic acid (30 µg); AMC, amoxycillin/clavulanic acid (30 µg); CAZ, ceftazidime (30 µg); IPM, imipenem (10 µg); ATM, aztreonam (30 µg); CTX, cefotaxime (30 µg); FOX, cefoxitin (30 µg); CN, gentamicin (10 µg); AK, amikacin (30 µg); STR, streptomycin (25 µg). Although MALDI-TOF mass spectrometry recognized all Cronobacter sakazakii and Cronobacter malonaticus strains, it could not identify Cronobacter muytjensii strain. The new species was placed in Enterobacter rather than Citrobacter because of its closer phenotypic and DNA similarity to E. cloacae, the type species of the genus Enterobacter, and because it was only 41% related by DNA hybridization to Citrobacter freundii, the types species of Citrobacter. isolates were pan-susceptible, continued antibiotic resistance surveillance is warranted. Free PMC article